Human F12(Coagulation factor XII) ELISA Kit

说明书
  • 规格
Product name

Human F12(Coagulation factor XII) ELISA Kit

Catalogue No.

EH1012

Alias

F12(Coagulation factor XII) ELISA Kit, HAEX ELISA Kit, Hageman factor ELISA Kit, HAE3 ELISA Kit, HAF ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

F12 ELISA Kit allows for the in vitro quantitative determination of F12 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

46.875-3000pg/ml

Sensitivity

< 28.125pg/ml

Standard curve

Human F12(Coagulation factor XII) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of F12 and the recovery rates were calculated by comparing the measured value to the expected amount of F12 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-104 97
EDTA plasma(n=5) 88-99 95
heparin plasma(n=5) 85-100 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-103% 88-101% 85-105% 86-99%
EDTA plasma(n=5) 89-99% 86-101% 85-100% 83-101%
heparin plasma(n=5) 86-97% 80-96% 80-100% 83-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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