Human CYB5A(Cytochrome b5) ELISA Kit

说明书
  • 规格
Product name

Human CYB5A(Cytochrome b5) ELISA Kit

Catalogue No.

EH1131

Alias

CYB5A(Cytochrome b5) ELISA Kit, CYB5 ELISA Kit, Microsomal cytochrome b5 type A ELISA Kit, MCB5 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

CYB5A ELISA Kit allows for the in vitro quantitative determination of CYB5A concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human CYB5A(Cytochrome b5) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CYB5A and the recovery rates were calculated by comparing the measured value to the expected amount of CYB5A in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-103 95
EDTA plasma(n=5) 85-105 98
heparin plasma(n=5) 94-103 99
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYB5A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-103% 86-104% 86-103% 87-100%
EDTA plasma(n=5) 83-97% 83-94% 85-93% 85-97%
heparin plasma(n=5) 80-99% 82-98% 87-100% 82-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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热线

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