Human CFD(Complement factor D) ELISA Kit

说明书
  • 规格
Product name

Human CFD(Complement factor D) ELISA Kit

Catalogue No.

EH0091

Alias

CFD ELISA Kit, ADN ELISA Kit, Adipsin ELISA Kit, DF ELISA Kit, PFD ELISA Kit, Factor D ELISA Kit, Properdin Factor D Esterase ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

CFD ELISA Kit allows for the in vitro quantitative determination of CFD concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human CFD(Complement factor D) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CFD and the recovery rates were calculated by comparing the measured value to the expected amount of CFD in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-103 97
EDTA plasma(n=5) 89-100 97
heparin plasma(n=5) 85-96 91
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CFD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-103% 85-102% 87-104% 89-105%
EDTA plasma(n=5) 84-95% 85-99% 87-98% 83-99%
heparin plasma(n=5) 85-100% 83-98% 82-100% 86-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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