Human CD8A(T-cell surface glycoprotein CD8 alpha chain) ELISA Kit

说明书
  • 规格
Product name

Human CD8A(T-cell surface glycoprotein CD8 alpha chain) ELISA Kit

Catalogue No.

EH1091

Alias

CD8A ELISA Kit, T-cell surface glycoprotein CD8 alpha chain ELISA Kit, T-lymphocyte differentiation antigen T8 ELISA Kit, Leu-2 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

CD8A ELISA Kit allows for the in vitro quantitative determination of CD8A concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

2.81-180ng/ml

Sensitivity

< 1.688ng/ml

Standard curve

Human CD8A(T-cell surface glycoprotein CD8 alpha chain) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CD8A and the recovery rates were calculated by comparing the measured value to the expected amount of CD8A in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-103 96
EDTA plasma(n=5) 85-102 92
heparin plasma(n=5) 85-103 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD8A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-104% 88-105% 86-105% 85-104%
EDTA plasma(n=5) 90-99% 83-85% 83-94% 86-98%
heparin plasma(n=5) 80-100% 89-98% 81-100% 81-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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