Human CD276(CD276 antigen) ELISA Kit

说明书
  • 规格
Product name

Human CD276(CD276 antigen) ELISA Kit

Catalogue No.

EH0634

Alias

CD276 ELISA Kit, B7-H3 ELISA Kit, B7H3 ELISA Kit, B7 homolog 3 ELISA Kit, CD276 antigen ELISA Kit, CD276 molecule ELISA Kit, Costimulatory molecule ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

CD276 ELISA Kit allows for the in vitro quantitative determination of CD276 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.781-50ng/ml

Sensitivity

< 0.469ng/ml

Standard curve

Human CD276(CD276 antigen) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CD276 and the recovery rates were calculated by comparing the measured value to the expected amount of CD276 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-100 94
EDTA plasma(n=5) 87-97 93
heparin plasma(n=5) 90-101 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD276 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-104% 87-104% 90-105% 85-105%
EDTA plasma(n=5) 83-99% 84-98% 84-96% 84-96%
heparin plasma(n=5) 84-100% 80-98% 83-98% 84-92%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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