Human C5a(Complement Component 5a) ELISA Kit

说明书
  • 规格
Product name

Human C5a(Complement Component 5a) ELISA Kit

Catalogue No.

EH0096

Alias

C5a(Complement Component 5a) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

C5a ELISA Kit allows for the in vitro quantitative determination of C5a concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78.125-5000pg/ml

Sensitivity

< 46.875pg/ml

Standard curve

Human C5a(Complement Component 5a) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of C5a and the recovery rates were calculated by comparing the measured value to the expected amount of C5a in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-96 91
EDTA plasma(n=5) 85-100 94
heparin plasma(n=5) 85-102 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C5a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-102% 90-105% 88-96% 90-99%
EDTA plasma(n=5) 88-100% 91-101% 82-92% 82-94%
heparin plasma(n=5) 80-99% 82-98% 88-100% 81-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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