Human BDH1(D-beta-hydroxybutyrate dehydrogenase, mitochondrial) ELISA Kit

说明书
  • 规格
Product name

Human BDH1(D-beta-hydroxybutyrate dehydrogenase, mitochondrial) ELISA Kit

Catalogue No.

EH0748

Alias

BDH1(D-beta-hydroxybutyrate dehydrogenase, mitochondrial) ELISA Kit, BDH ELISA Kit, 3-hydroxybutyrate dehydrogenase ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

BDH1 ELISA Kit allows for the in vitro quantitative determination of BDH1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.2-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human BDH1(D-beta-hydroxybutyrate dehydrogenase, mitochondrial) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of BDH1 and the recovery rates were calculated by comparing the measured value to the expected amount of BDH1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-103 98
EDTA plasma(n=5) 85-103 96
heparin plasma(n=5) 87-104 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BDH1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-101% 93-104% 87-105% 87-99%
EDTA plasma(n=5) 87-97% 82-96% 83-100% 82-99%
heparin plasma(n=5) 80-99% 88-96% 82-96% 82-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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