Human BAFF(B-Cell Activating Factor) ELISA Kit

说明书
  • 规格
Product name

Human BAFF(B-Cell Activating Factor) ELISA Kit

Catalogue No.

EH0042

Alias

BAFF ELISA Kit, CD257 ELISA Kit, TNFSF13B ELISA Kit, BLYS ELISA Kit, TALL1 ELISA Kit, THANK ELISA Kit, TNFSF20 ELISA Kit, ZTNF4 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

BAFF ELISA Kit allows for the in vitro quantitative determination of BAFF concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

62.5-4000pg/ml

Sensitivity

< 37.5pg/ml

Standard curve

Human BAFF(B-Cell Activating Factor) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of BAFF and the recovery rates were calculated by comparing the measured value to the expected amount of BAFF in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-103 95
EDTA plasma(n=5) 92-102 96
heparin plasma(n=5) 87-104 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BAFF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-101% 88-104% 94-105% 93-104%
EDTA plasma(n=5) 87-98% 82-98% 82-101% 86-101%
heparin plasma(n=5) 80-87% 83-88% 83-96% 85-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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