Human ASPN(Asporin) ELISA Kit

说明书
  • 规格
Product name

Human ASPN(Asporin) ELISA Kit

Catalogue No.

EH1174

Alias

ASPN ELISA Kit, OS3 ELISA Kit, PLAP-1 ELISA Kit, PLAP1 ELISA Kit, SLRR1C ELISA Kit, Periodontal ligament-associated protein 1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

ASPN ELISA Kit allows for the in vitro quantitative determination of ASPN concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human ASPN(Asporin) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ASPN and the recovery rates were calculated by comparing the measured value to the expected amount of ASPN in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-102 95
EDTA plasma(n=5) 92-105 96
heparin plasma(n=5) 88-100 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ASPN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-102% 87-95% 95-104% 86-103%
EDTA plasma(n=5) 86-93% 85-101% 84-100% 82-101%
heparin plasma(n=5) 82-89% 83-100% 87-93% 82-94%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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