Human AFP-L3(Alpha-fetoprotein Lens culinaris agglutiin 3) ELISA Kit

说明书
  • 规格
Product name

Human AFP-L3(Alpha-fetoprotein Lens culinaris agglutiin 3) ELISA Kit

Catalogue No.

EH1268

Alias

AFP-L(Alpha-fetoprotein Lens culinaris agglutiin 3) ELISA Kit, AFPL3 ELISA Kit, aFPL3 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

AFP-L3 ELISA Kit allows for the in vitro quantitative determination of AFP-L3 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.625-40ng/ml

Sensitivity

< 0.375ng/ml

Standard curve

Human AFP-L3(Alpha-fetoprotein Lens culinaris agglutiin 3) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of AFP-L3 and the recovery rates were calculated by comparing the measured value to the expected amount of AFP-L3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-105 95
EDTA plasma(n=5) 92-100 96
heparin plasma(n=5) 85-100 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AFP-L3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-103% 85-104% 86-104% 91-105%
EDTA plasma(n=5) 82-101% 82-101% 82-96% 82-99%
heparin plasma(n=5) 81-100% 85-99% 88-100% 80-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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