Human ACAT1(Acetyl-CoA acetyltransferase, mitochondrial) ELISA Kit

说明书
  • 规格
Product name

Human ACAT1(Acetyl-CoA acetyltransferase, mitochondrial) ELISA Kit

Catalogue No.

EH0798

Alias

ACAT1 ELISA Kit, AACAT ELISA Kit, MAT ELISA Kit, Acetyl-CoA acetyltransferase, mitochondrial ELISA Kit, Acetoacetyl-CoA thiolase ELISA Kit, T2 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

ACAT1 ELISA Kit allows for the in vitro quantitative determination of ACAT1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

7.8-500pg/ml

Sensitivity

< 4.688pg/ml

Standard curve

Human ACAT1(Acetyl-CoA acetyltransferase, mitochondrial) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ACAT1 and the recovery rates were calculated by comparing the measured value to the expected amount of ACAT1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-105 95
EDTA plasma(n=5) 88-104 95
heparin plasma(n=5) 86-102 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACAT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-105% 90-103% 86-94% 86-105%
EDTA plasma(n=5) 84-96% 85-95% 85-94% 90-101%
heparin plasma(n=5) 82-99% 81-95% 81-100% 81-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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