Sample Processing is to collect target protein. The processing shall be done as gently as possible as protein denatures or degrades easily. It is also very important to store processed sample, especially avoiding repeated free-thaw cycles. Processed sample can be sub-packed and sealed. Expiry Time: a week at 4 degree; a month at -20 degree; two months at -80 degree. Just put sample in room until its temperature reaches room temperature before using the sample. It is forbidden to heat the sample to melt the sample.
Set at 4 degree, and centrifuge sample at 12000g for 30 minutes. Remove upper grease and downer sediment, and then collect middle part for test.