Products
MGEA5 antibody
- Product Name
- MGEA5 antibody
- Catalogue No.
- FNab05164
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG2a
- Clone ID
- 9B9
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- meningioma expressed antigen 5(hyaluronidase)
- Alternative Names
- HEXC antibody, KIAA0679 antibody, MEA5 antibody
- UniProt ID
- O60502
- Observed MW
- 130 kDa
Application
- Tested Applications
- ELISA, WB, IF, IHC
- Recommended dilution
- WB: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
Human brain lysate were subjected to SDS PAGE followed by western blot with FNab05164(MGEA5 antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human gliomas using FNab05164(MGEA5 antibody) at dilution of 1:50
- Background
- Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc(in vitro)(PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity(PubMed:24088714). Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc(in vitro), but has about six times lower specific activity than isoform 1.
