Products
AFF1 antibody
Category:
Research Area:
- SPECIFICATIONS
- Product Name
- AFF1 antibody
- Catalogue No.
- FNab00195
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- AF4/FMR2 family, member 1
- Alternative Names
- AFF1 antibody, MLLT2 antibody, AF4 MLL antibody, PBM1 antibody, AF 4 antibody
- UniProt ID
- P51825
- Observed MW
- 131 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:100
Validated Images
K-562 cells were subjected to SDS PAGE followed by western blot with FNab00195(AFF1-Specific antibody) at dilution of 1:1000
Immunofluorescence analysis of U2OS cells using FNab00195(AFF1-Specific antibody).
- Background
- This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants.