Products

Mouse FVIII(Coagulation Factor VIII)ELISA Kit

EM1043 Catalogue No.
FVIII ELISA Kit, F8 ELISA Kit
Reactivity
Mouse
Range
15.625-1000ng/ml
Sensitivity
9.375ng/ml
Product Name
Mouse FVIII(Coagulation Factor VIII)ELISA Kit
Catalogue No.
EM1043
Alias
FVIII ELISA Kit, F8 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
FVIIIELISA Kit allows for the in vitro quantitative determination of FVIII concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000ng/ml
Sensitivity
< 9.375ng/ml
Species
Mouse
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of FVIII and the recovery rates were calculated by comparing the measured value to the expected amount of FVIII in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10392
EDTA plasma(n=5)91-10496
heparin plasma(n=5)89-10497
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FVIII and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)85-96%87-103%89-104%85-105%
EDTA plasma(n=5)82-101%83-101%83-93%85-99%
heparin plasma(n=5)80-99%82-99%88-99%83-95%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only