News

Dealing Stool (feces) sample in ELISA testing

Time: 2017-04-01 14:30:38

Dealing Stool  (feces) sample in ELISA testing

www.fn-test.com 2017-04-01


Dealing Stool sample by ethanol in ELISA testing

Taking Stool sample 0.2-0.5g, adding 0.5ml ethanol and repeat pipetting for 1 minute, put it under room temperature(20-25) for 10 minutes, then centrifuge the mixture under room temperature, running at 8000-10000 RPM for 10 mins, then drain the supernate into a clean PE tube. Adding 100 ul supernate into the 900ul sample dilution, then
pipet 50 ul sample and 50 ul detection antibody to each well, then proceed testing according to the procedures stated in the manual.

Dealing Stool sample by PBS in ELISA Testing

Taking Stool sample 0.2-0.5g, adding 0.5ml PBS and repeat pipetting for 1 minute, put it under room temperature(20-25) for 10 minutes, then centrifuge the mixture under room temperature, running at 8000-10000 RPM for 10 mins, then drain the supernate into a clean PE tube. To add 50 ul supernate and 50 ul detection antibody to each well, then proceed testing according to the procedures stated in the manual.

To dissolve the Stool in ethanol(chemical purityat 99%, 100% concentration), centrifuge the mixture under room temperature, running at 8000-10000RPM for 10 mins, and filter the solid particles from the mixture, getting the supernate, adding 10 ul supernate into the 90ul sample dilution, then proceed testing.

Service

点击这里给我发消息

Hotline

027-87384275