Solution for ELISA Kits Poor Standard Curve
1.Improper standard solution
Confirm dilutions are made correctly.
2.Standard improperly reconstituted
Briefly spin vial before opening; inspect for undissolved material after reconstituting.
Store and handle standard as recommended.
4.Curve doesn't fit scale
Try plotting using different scales e.g. log-log, 5parameter logistic curve fit.
Use calibrated pipettes and properpipetting technique.
6.Standard was incompletely reconstituted or wasinappropriately stored
Reconstitute standard according to protocol. Store reconstituted standard in appropriate vials.Store reconstituted standard at -70℃
7.Reagents added to wells with incorrect concentrations
Check for pipetting errors and correct reagent volume.
8.Incubations done at inapproprite temperature,timing or agitation
Assay conditions need to be checked.