5-HETE(5-Hydroxyeicosatetraenoic Acid) ELISA Kit

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Product name

5-HETE(5-Hydroxyeicosatetraenoic Acid) ELISA Kit

Catalogue No.

EU0185

Detection method

Competitive ELISA, Coated with Antigen

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of and the recovery rates were calculated by comparing the measured value to the expected amount of in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)89-10498
EDTA plasma(n=5)85-9992
heparin plasma(n=5)88-10597
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)87-101%85-104%86-99%88-102%
EDTA plasma(n=5)82-93%83-100%82-95%84-98%
heparin plasma(n=5)93-100%83-99%83-99%83-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Current product's manual: Manual

Protocol may be updated. Please feel free to contact Finetest Team to acquire the latest version.

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