Rat TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) ELISA Kit

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  • Specifications
Product name

Rat TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) ELISA Kit

Catalogue No.

ER1402

Detection method

Sandwich ELISA, Double Antibody

Application

TRAIL/TNFSF10 ELISA Kit allows for the in vitro quantitative determination of TRAIL/TNFSF10 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of TRAIL/TNFSF10 and the recovery rates were calculated by comparing the measured value to the expected amount of TRAIL/TNFSF10 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-101 94
EDTA plasma(n=5) 86-105 95
heparin plasma(n=5) 88-103 98
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRAIL/TNFSF10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-105% 96-103% 87-98% 93-101%
EDTA plasma(n=5) 85-101% 82-100% 85-99% 88-101%
heparin plasma(n=5) 80-99% 80-99% 85-100% 80-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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