Rat SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit

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  • Specifications
Product name

Rat SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit

Catalogue No.

ER1353

Detection method

Sandwich ELISA, Double Antibody

Application

SPD ELISA Kit allows for the in vitro quantitative determination of SPD concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78.125-5000pg/ml

Sensitivity

< 46.875pg/ml

Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of SPD and the recovery rates were calculated by comparing the measured value to the expected amount of SPD in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-104 94
EDTA plasma(n=5) 85-102 95
heparin plasma(n=5) 88-104 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-102% 87-102% 85-97% 85-99%
EDTA plasma(n=5) 87-101% 84-99% 86-101% 85-98%
heparin plasma(n=5) 85-96% 84-96% 84-100% 82-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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