Rat SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit

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Product name

Rat SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit

Catalogue No.

ER1353

Detection method

Sandwich ELISA, Double Antibody

Size

96T

Range

78.125-5000pg/ml

Sensitivity

< 46.875pg/ml

Standard curve
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of SPD and the recovery rates were calculated by comparing the measured value to the expected amount of SPD in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)88-10494
EDTA plasma(n=5)85-10295
heparin plasma(n=5)88-10493
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)85-102%87-102%85-97%85-99%
EDTA plasma(n=5)87-101%84-99%86-101%85-98%
heparin plasma(n=5)85-96%84-96%84-100%82-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Current product's manual: Manual

Protocol may be updated. Please feel free to contact Finetest Team to acquire the latest version.

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