Rat IL-6(Interleukin-6) ELISA Kit

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Product name

Rat IL-6(Interleukin-6) ELISA Kit

Catalogue No.

ER0042

Detection method

Sandwich ELISA, Double Antibody

Size

96T

Range

62.5-4000pg/ml

Sensitivity

< 37.5pg/ml

Standard curve
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-6 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)91-9795
EDTA plasma(n=5)85-10595
heparin plasma(n=5)89-10395
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)88-104%85-105%92-103%90-105%
EDTA plasma(n=5)82-100%83-99%83-100%83-99%
heparin plasma(n=5)80-100%84-96%82-96%85-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Current product's manual: Manual

Protocol may be updated. Please feel free to contact Finetest Team to acquire the latest version.

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