Rat HSP-40 (Heat Shock Protein 40) ELISA Kit

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  • Specifications
Product name

Rat HSP-40 (Heat Shock Protein 40) ELISA Kit

Catalogue No.

ER1054

Detection method

Sandwich ELISA, Double Antibody

Application

HSP-40 ELISA Kit allows for the in vitro quantitative determination of HSP-40 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of HSP-40 and the recovery rates were calculated by comparing the measured value to the expected amount of HSP-40 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-95 90
EDTA plasma(n=5) 85-100 91
heparin plasma(n=5) 90-103 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSP-40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-100% 85-98% 88-104% 86-104%
EDTA plasma(n=5) 85-101% 82-100% 83-99% 82-99%
heparin plasma(n=5) 80-97% 80-100% 81-94% 82-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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