Rat HIF-1α (Hypoxia-inducible factor 1-alpha) ELISA Kit

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Product name

Rat HIF-1α (Hypoxia-inducible factor 1-alpha) ELISA Kit

Catalogue No.

ER0191

Detection method

Sandwich ELISA, Double Antibody

Size

96T

Range

7.813-500pg/ml

Sensitivity

< 4.688pg/ml

Standard curve
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of HIF-1α and the recovery rates were calculated by comparing the measured value to the expected amount of HIF-1α in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)86-10093
EDTA plasma(n=5)86-9891
heparin plasma(n=5)88-10291
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIF-1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)94-99%92-103%90-100%86-105%
EDTA plasma(n=5)82-94%83-101%82-94%82-94%
heparin plasma(n=5)82-91%85-98%87-97%83-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Current product's manual: Manual

Protocol may be updated. Please feel free to contact Finetest Team to acquire the latest version.

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027-87384275
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