Rat HIF-1α (Hypoxia-inducible factor 1-alpha) ELISA Kit

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  • Specifications
Product name

Rat HIF-1α (Hypoxia-inducible factor 1-alpha) ELISA Kit

Catalogue No.

ER0191

Detection method

Sandwich ELISA, Double Antibody

Application

HIF-1α ELISA Kit allows for the in vitro quantitative determination of HIF-1α concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

7.813-500pg/ml

Sensitivity

< 4.688pg/ml

Standard curve

Rat HIF-1α (Hypoxia-inducible factor 1-alpha) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of HIF-1α and the recovery rates were calculated by comparing the measured value to the expected amount of HIF-1α in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-100 93
EDTA plasma(n=5) 86-98 91
heparin plasma(n=5) 88-102 91
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIF-1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-99% 92-103% 90-100% 86-105%
EDTA plasma(n=5) 82-94% 83-101% 82-94% 82-94%
heparin plasma(n=5) 82-91% 85-98% 87-97% 83-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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