Rat GATA3 (GATA Binding Protein 3) ELISA Kit

Manual
  • Specifications
Product name

Rat GATA3 (GATA Binding Protein 3) ELISA Kit

Catalogue No.

ER0978

Detection method

Sandwich ELISA, Double Antibody

Application

GATA3 ELISA Kit allows for the in vitro quantitative determination of GATA3 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Rat GATA3 (GATA Binding Protein 3) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of GATA3 and the recovery rates were calculated by comparing the measured value to the expected amount of GATA3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-103 97
EDTA plasma(n=5) 87-101 94
heparin plasma(n=5) 88-99 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GATA3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-105% 91-101% 88-105% 85-95%
EDTA plasma(n=5) 82-99% 83-99% 83-99% 82-99%
heparin plasma(n=5) 80-99% 85-92% 85-96% 85-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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