Mouse TNFα(Tumor Necrosis Factor Alpha) ELISA Kit

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Product name

Mouse TNFα(Tumor Necrosis Factor Alpha) ELISA Kit

Catalogue No.

EM0183

Alias

TNFα ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of TNFα (Tumor Necrosis Factor Alpha) /TNF-α/DIF/TNF-alpha/TNFA/TNFSF2 and the recovery rates were calculated by comparing the measured value to the expected amount of TNFα (Tumor Necrosis Factor Alpha) /TNF-α/DIF/TNF-alpha/TNFA/TNFSF2 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10494
EDTA plasma(n=5)86-10397
heparin plasma(n=5)91-10296
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFα (Tumor Necrosis Factor Alpha) /TNF-α/DIF/TNF-alpha/TNFA/TNFSF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)93-102%90-105%85-100%87-102%
EDTA plasma(n=5)86-101%82-99%82-93%89-101%
heparin plasma(n=5)80-96%82-99%81-89%85-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Current product's manual: Manual

Protocol may be updated. Please feel free to contact Finetest Team to acquire the latest version.

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