Mouse TNFα(Tumor Necrosis Factor Alpha) ELISA Kit

Manual
  • Specifications
Product name

Mouse TNFα(Tumor Necrosis Factor Alpha) ELISA Kit

Catalogue No.

EM0183

Alias

TNFα ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

TNFα ELISA Kit allows for the in vitro quantitative determination of TNFα(Tumor Necrosis Factor Alpha)/TNF-α/DIF/TNF-alpha/TNFA/TNFSF2 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Mouse TNFα(Tumor Necrosis Factor Alpha) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of TNFα(Tumor Necrosis Factor Alpha)/TNF-α/DIF/TNF-alpha/TNFA/TNFSF2 and the recovery rates were calculated by comparing the measured value to the expected amount of TNFα(Tumor Necrosis Factor Alpha)/TNF-α/DIF/TNF-alpha/TNFA/TNFSF2 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-104 94
EDTA plasma(n=5) 86-103 97
heparin plasma(n=5) 91-102 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFα(Tumor Necrosis Factor Alpha)/TNF-α/DIF/TNF-alpha/TNFA/TNFSF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-102% 90-105% 85-100% 87-102%
EDTA plasma(n=5) 86-101% 82-99% 82-93% 89-101%
heparin plasma(n=5) 80-96% 82-99% 81-89% 85-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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