Mouse RasGAP (Ras GTPase Activating Protein) ELISA Kit

Manual
  • Specifications
Product name

Mouse RasGAP (Ras GTPase Activating Protein) ELISA Kit

Catalogue No.

EM1330

Alias

RasGAP ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

RasGAP ELISA Kit allows for the in vitro quantitative determination of RasGAP/p195/SAR1/HUMORFA01/IQ motif containing GTPase activating protein 1/KIAA0051/ras GTPase-activating-like protein IQGAP1/RasGAP-like with IQ motifs concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78.125-5000pg/ml

Sensitivity

< 46.875pg/ml

Standard curve

Mouse RasGAP (Ras GTPase Activating Protein) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of RasGAP/p195/SAR1/HUMORFA01/IQ motif containing GTPase activating protein 1/KIAA0051/ras GTPase-activating-like protein IQGAP1/RasGAP-like with IQ motifs and the recovery rates were calculated by comparing the measured value to the expected amount of RasGAP/p195/SAR1/HUMORFA01/IQ motif containing GTPase activating protein 1/KIAA0051/ras GTPase-activating-like protein IQGAP1/RasGAP-like with IQ motifs in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-103 93
EDTA plasma(n=5) 85-97 92
heparin plasma(n=5) 87-103 98
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RasGAP/p195/SAR1/HUMORFA01/IQ motif containing GTPase activating protein 1/KIAA0051/ras GTPase-activating-like protein IQGAP1/RasGAP-like with IQ motifs and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-104% 93-105% 86-103% 85-91%
EDTA plasma(n=5) 83-96% 85-97% 94-101% 88-97%
heparin plasma(n=5) 89-97% 82-97% 83-97% 82-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Service

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Hotline

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