Mouse PAR2 (Protease Activated Receptor 2) ELISA Kit

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Product name

Mouse PAR2 (Protease Activated Receptor 2) ELISA Kit

Catalogue No.

EM1265

Alias

PAR2 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CFIIRL1/F2RL1/GPCR11/CFIIRL1/coagulation factor II (thrombin) receptor-like 1/Coagulation factor II receptor-like 1/F2RL1/GPCR11/GPR11protease-activated receptor 2/G-protein coupled receptor 11/PAR-2/PAR2G protein-coupled receptor-11/proteinase-activated receptor 2/proteinase-activated receptor-2/Thrombin receptor-like 1 and the recovery rates were calculated by comparing the measured value to the expected amount of CFIIRL1/F2RL1/GPCR11/CFIIRL1/coagulation factor II (thrombin) receptor-like 1/Coagulation factor II receptor-like 1/F2RL1/GPCR11/GPR11protease-activated receptor 2/G-protein coupled receptor 11/PAR-2/PAR2G protein-coupled receptor-11/proteinase-activated receptor 2/proteinase-activated receptor-2/Thrombin receptor-like 1 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)86-9891
EDTA plasma(n=5)89-9692
heparin plasma(n=5)87-9892
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CFIIRL1/F2RL1/GPCR11/CFIIRL1/coagulation factor II (thrombin) receptor-like 1/Coagulation factor II receptor-like 1/F2RL1/GPCR11/GPR11protease-activated receptor 2/G-protein coupled receptor 11/PAR-2/PAR2G protein-coupled receptor-11/proteinase-activated receptor 2/proteinase-activated receptor-2/Thrombin receptor-like 1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)86-104%85-103%90-105%89-105%
EDTA plasma(n=5)86-95%82-99%82-93%88-100%
heparin plasma(n=5)83-98%81-99%84-97%86-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Current product's manual: Manual

Protocol may be updated. Please feel free to contact Finetest Team to acquire the latest version.

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