Mouse NF-κB p65 (Nuclear Factor-κB p65) ELISA Kit

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Product name

Mouse NF-κB p65 (Nuclear Factor-κB p65) ELISA Kit

Catalogue No.

EM1231

Alias

NF-κB p65 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of NF-κB p65/NFkB p65/NFKB3/p65RelA/MGC131774/NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65) ) /Nuclear factor NF-kappa-B p65 subunit/Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65/p65/v-rel reticuloendotheliosis viral oncogene homolog A (avian) /v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65 and the recovery rates were calculated by comparing the measured value to the expected amount of NF-κB p65/NFkB p65/NFKB3/p65RelA/MGC131774/NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65) ) /Nuclear factor NF-kappa-B p65 subunit/Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65/p65/v-rel reticuloendotheliosis viral oncogene homolog A (avian) /v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)88-10594
EDTA plasma(n=5)88-9490
heparin plasma(n=5)87-10597
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NF-κB p65/NFkB p65/NFKB3/p65RelA/MGC131774/NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65) ) /Nuclear factor NF-kappa-B p65 subunit/Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65/p65/v-rel reticuloendotheliosis viral oncogene homolog A (avian) /v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)86-104%87-102%89-104%87-103%
EDTA plasma(n=5)82-100%84-100%84-99%82-95%
heparin plasma(n=5)80-100%84-94%83-100%85-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Current product's manual: Manual

Protocol may be updated. Please feel free to contact Finetest Team to acquire the latest version.

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