Mouse IL-1b(Interleukin 1 Beta) ELISA Kit

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Product name

Mouse IL-1b(Interleukin 1 Beta) ELISA Kit

Catalogue No.

EM0109

Alias

IL-1b ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Size

96T

Range

12.5-800pg/ml

Sensitivity

< 7.5pg/ml

Standard curve
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-1β (Interleukin 1 Beta) /IL-1beta/IL1B/IL1-BETA/IL1F2/catabolin and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1β (Interleukin 1 Beta) /IL-1beta/IL1B/IL1-BETA/IL1F2/catabolin in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)91-10296
EDTA plasma(n=5)88-10494
heparin plasma(n=5)89-10397
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1β (Interleukin 1 Beta) /IL-1beta/IL1B/IL1-BETA/IL1F2/catabolin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)90-104%88-103%86-104%98-101%
EDTA plasma(n=5)82-100%89-101%84-98%86-101%
heparin plasma(n=5)84-99%80-94%84-95%82-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Current product's manual: Manual

Protocol may be updated. Please feel free to contact Finetest Team to acquire the latest version.

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