Mouse Hif1α(Hypoxia-inducible factor 1-alpha) ELISA Kit

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Product name

Mouse Hif1α(Hypoxia-inducible factor 1-alpha) ELISA Kit

Catalogue No.

EM0310

Alias

Hif1α ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Size

96T

Range

125-8000pg/ml

Sensitivity

< 75pg/ml

Standard curve
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of Hif1α/HIF-1α/HIF1A/ARNT-interacting protein/Basic-helix-loop-helix-PAS protein MOP1/BHLHE78/Class E basic helix-loop-helix protein 78/HIF-1-alpha/HIF1-alpha/hypoxia-inducible factor 1-alpha/Member of PAS protein 1/member of PAS superfamily 1/PAS domain-containing protein 8 and the recovery rates were calculated by comparing the measured value to the expected amount of Hif1α/HIF-1α/HIF1A/ARNT-interacting protein/Basic-helix-loop-helix-PAS protein MOP1/BHLHE78/Class E basic helix-loop-helix protein 78/HIF-1-alpha/HIF1-alpha/hypoxia-inducible factor 1-alpha/Member of PAS protein 1/member of PAS superfamily 1/PAS domain-containing protein 8 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)87-10094
EDTA plasma(n=5)93-10499
heparin plasma(n=5)92-10298
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hif1α/HIF-1α/HIF1A/ARNT-interacting protein/Basic-helix-loop-helix-PAS protein MOP1/BHLHE78/Class E basic helix-loop-helix protein 78/HIF-1-alpha/HIF1-alpha/hypoxia-inducible factor 1-alpha/Member of PAS protein 1/member of PAS superfamily 1/PAS domain-containing protein 8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)87-96%88-100%92-102%89-98%
EDTA plasma(n=5)85-98%86-97%82-101%94-100%
heparin plasma(n=5)80-97%80-99%80-99%83-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Current product's manual: Manual

Protocol may be updated. Please feel free to contact Finetest Team to acquire the latest version.

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