Mouse FVIII (Coagulation Factor VIII) ELISA Kit

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Product name

Mouse FVIII (Coagulation Factor VIII) ELISA Kit

Catalogue No.

EM1043

Alias

FVIII ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of FVIII/F8 and the recovery rates were calculated by comparing the measured value to the expected amount of FVIII/F8 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)86-10391
EDTA plasma(n=5)90-10297
heparin plasma(n=5)86-10394
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FVIII/F8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)94-100%89-101%90-105%89-104%
EDTA plasma(n=5)87-97%82-94%93-97%83-95%
heparin plasma(n=5)84-93%85-96%82-96%80-89%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Current product's manual: Manual

Protocol may be updated. Please feel free to contact Finetest Team to acquire the latest version.

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