Mouse FVIII (Coagulation Factor VIII) ELISA Kit

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  • Specifications
Product name

Mouse FVIII (Coagulation Factor VIII) ELISA Kit

Catalogue No.

EM1043

Alias

FVIII ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

FVIII ELISA Kit allows for the in vitro quantitative determination of FVIII/F8 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of FVIII/F8 and the recovery rates were calculated by comparing the measured value to the expected amount of FVIII/F8 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-103 91
EDTA plasma(n=5) 90-102 97
heparin plasma(n=5) 86-103 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FVIII/F8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-100% 89-101% 90-105% 89-104%
EDTA plasma(n=5) 87-97% 82-94% 93-97% 83-95%
heparin plasma(n=5) 84-93% 85-96% 82-96% 80-89%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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