Human TNFα(Tumor Necrosis Factor Alpha) ELISA Kit

Manual
  • Specifications
Product name

Human TNFα(Tumor Necrosis Factor Alpha) ELISA Kit

Catalogue No.

EH0302

Alias

TNFα(Tumor Necrosis Factor Alpha) ELISA Kit, TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

TNFα ELISA Kit allows for the in vitro quantitative determination of TNFα concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.6-1000pg/ml

Sensitivity

< 1 pg/ml

Standard curve

Human TNFα(Tumor Necrosis Factor Alpha) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of TNFα and the recovery rates were calculated by comparing the measured value to the expected amount of TNFα in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-105 95
EDTA plasma(n=5) 88-103 94
heparin plasma(n=5) 89-104 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-104% 90-104% 90-103% 86-105%
EDTA plasma(n=5) 85-101% 82-101% 91-101% 83-101%
heparin plasma(n=5) 82-99% 81-98% 81-100% 86-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Service

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Hotline

027-87384275
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