Human TNFα(Tumor Necrosis Factor Alpha) ELISA Kit

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Product name

Human TNFα(Tumor Necrosis Factor Alpha) ELISA Kit

Catalogue No.

EH0302

Alias

TNFα (Tumor Necrosis Factor Alpha) ELISA Kit, TNF-α ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Size

96T

Range

15.6-1000pg/ml

Sensitivity

< 1 pg/ml

Standard curve
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of TNFα and the recovery rates were calculated by comparing the measured value to the expected amount of TNFα in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)86-10595
EDTA plasma(n=5)88-10394
heparin plasma(n=5)89-10497
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)87-104%90-104%90-103%86-105%
EDTA plasma(n=5)85-101%82-101%91-101%83-101%
heparin plasma(n=5)82-99%81-98%81-100%86-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Current product's manual: Manual

Protocol may be updated. Please feel free to contact Finetest Team to acquire the latest version.

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027-87384275
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