Human SLC12A1(Solute carrier family 12 member 1) ELISA Kit

Manual
  • Specifications
Product name

Human SLC12A1(Solute carrier family 12 member 1) ELISA Kit

Catalogue No.

EH0836

Alias

SLC12A1(Solute carrier family 12 member 1) ELISA Kit, NKCC2 ELISA Kit, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 ELISA Kit, Kidney-specific Na-K-Cl symporter ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

SLC12A1 ELISA Kit allows for the in vitro quantitative determination of SLC12A1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.312-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human SLC12A1(Solute carrier family 12 member 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of SLC12A1 and the recovery rates were calculated by comparing the measured value to the expected amount of SLC12A1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-102 91
EDTA plasma(n=5) 85-96 94
heparin plasma(n=5) 86-96 91
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SLC12A1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-103% 88-100% 87-92% 86-95%
EDTA plasma(n=5) 85-92% 87-97% 84-92% 82-93%
heparin plasma(n=5) 85-97% 82-100% 81-96% 85-92%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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