Human SIRT5(NAD-dependent deacetylase sirtuin-5) ELISA Kit

Manual
  • Specifications
Product name

Human SIRT5(NAD-dependent deacetylase sirtuin-5) ELISA Kit

Catalogue No.

EH1295

Alias

SIRT5(NAD-dependent deacetylase sirtuin-5) ELISA Kit, SIR2L5 ELISA Kit, SIRT5 ELISA Kit, silent mating type information regulation 2, S.cerevisiae, homolog 5 ELISA Kit, sir2-like 5 ELISA Kit, SIR2-like protein 5 ELISA Kit, sirtuin 5 ELISA Kit, sirtuin type 5 ELISA Kit, Regulatory protein SIR2 homolog 5 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

SIRT5 ELISA Kit allows for the in vitro quantitative determination of SIRT5 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human SIRT5(NAD-dependent deacetylase sirtuin-5) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of SIRT5 and the recovery rates were calculated by comparing the measured value to the expected amount of SIRT5 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-105 100
EDTA plasma(n=5) 86-102 93
heparin plasma(n=5) 89-103 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SIRT5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-100% 89-105% 96-103% 97-104%
EDTA plasma(n=5) 82-100% 86-101% 85-100% 83-98%
heparin plasma(n=5) 83-97% 81-94% 83-97% 80-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Service

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Hotline

027-87384275
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