Human SESN3(Sestrin-3) ELISA Kit

Manual
  • Specifications
Product name

Human SESN3(Sestrin-3) ELISA Kit

Catalogue No.

EH0829

Alias

SESN3(Sestrin-3) ELISA Kit, SEST3 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

SESN3 ELISA Kit allows for the in vitro quantitative determination of SESN3 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human SESN3(Sestrin-3) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of SESN3 and the recovery rates were calculated by comparing the measured value to the expected amount of SESN3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-103 94
EDTA plasma(n=5) 86-102 94
heparin plasma(n=5) 85-103 91
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SESN3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-103% 89-97% 86-103% 86-102%
EDTA plasma(n=5) 84-101% 87-98% 82-94% 86-100%
heparin plasma(n=5) 82-96% 82-100% 92-99% 82-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Service

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Hotline

027-87384275
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