Human S100A1/S100(Protein S100-A1) ELISA Kit

Manual
  • Specifications
Product name

Human S100A1/S100(Protein S100-A1) ELISA Kit

Catalogue No.

EH0380

Alias

S100A1 ELISA Kit, S100A ELISA Kit, S100 calcium-binding protein A1 ELISA Kit, protein S100-A1 ELISA Kit, S100 alpha ELISA Kit, S100 calcium-binding protein A1S100 ELISA Kit, S-100 protein alpha chain ELISA Kit, S-100 protein subunit alpha ELISA Kit, S100 protein, alpha polypeptide ELISA Kit, S100-alpha ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

S100A1/S100 ELISA Kit allows for the in vitro quantitative determination of S100A1/S100 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human S100A1/S100(Protein S100-A1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of S100A1/S100 and the recovery rates were calculated by comparing the measured value to the expected amount of S100A1/S100 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-102 94
EDTA plasma(n=5) 87-103 96
heparin plasma(n=5) 85-105 98
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S100A1/S100 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-105% 88-104% 87-103% 89-97%
EDTA plasma(n=5) 85-100% 84-100% 82-94% 84-99%
heparin plasma(n=5) 82-99% 80-99% 82-98% 82-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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