Human PIIINP(N-terminal propeptide of Collagen alpha-1(III) chain) ELISA Kit

Manual
  • Specifications
Product name

Human PIIINP(N-terminal propeptide of Collagen alpha-1(III) chain) ELISA Kit

Catalogue No.

EH0960

Alias

PIIINP ELISA Kit, P3NP ELISA Kit, N-Propeptide Of Type III Procollagen ELISA Kit, Procollagen III Amino Terminal Propeptide ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

PIIINP chain) ELISA Kit allows for the in vitro quantitative determination of PIIINP concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

23.438-1500pg/ml

Sensitivity

< 14.063pg/ml

Standard curve

Human PIIINP(N-terminal propeptide of Collagen alpha-1(III) chain) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PIIINP and the recovery rates were calculated by comparing the measured value to the expected amount of PIIINP in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-105 98
EDTA plasma(n=5) 90-103 95
heparin plasma(n=5) 89-104 98
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIIINP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-105% 86-105% 85-104% 86-99%
EDTA plasma(n=5) 83-95% 82-95% 82-96% 90-100%
heparin plasma(n=5) 80-93% 81-99% 80-99% 85-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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