Human PF4(Platelet factor 4) ELISA Kit

Manual
  • Specifications
Product name

Human PF4(Platelet factor 4) ELISA Kit

Catalogue No.

EH0744

Alias

CXCL4 ELISA Kit, PF4(Platelet Factor 4) ELISA Kit, chemokine(C-X-C motif) ligand 4 ELISA Kit, C-X-C motif chemokine 4 ELISA Kit, Oncostatin-A ELISA Kit, PF-4 ELISA Kit, platelet factor 4 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

PF4 ELISA Kit allows for the in vitro quantitative determination of PF4 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

1.563-100ng/ml

Sensitivity

< 0.938ng/ml

Standard curve

Human PF4(Platelet factor 4) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PF4 and the recovery rates were calculated by comparing the measured value to the expected amount of PF4 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-103 95
EDTA plasma(n=5) 86-105 99
heparin plasma(n=5) 92-103 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PF4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-103% 87-102% 91-102% 89-103%
EDTA plasma(n=5) 95-100% 84-100% 83-101% 87-100%
heparin plasma(n=5) 81-99% 81-97% 89-99% 82-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Service

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Hotline

027-87384275
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