Human PECAM-1(Platelet/Endothelial Cell Adhesion Molecule 1) ELISA Kit

Manual
  • Specifications
Product name

Human PECAM-1(Platelet/Endothelial Cell Adhesion Molecule 1) ELISA Kit

Catalogue No.

EH0021

Alias

CD31 ELISA Kit, PECAM-1 ELISA Kit, (Platelet ELISA Kit, Endothelial Cell Adhesion Molecule 1) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

PECAM-1 ELISA Kit allows for the in vitro quantitative determination of PECAM-1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human PECAM-1(Platelet/Endothelial Cell Adhesion Molecule 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PECAM-1 and the recovery rates were calculated by comparing the measured value to the expected amount of PECAM-1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-103 98
EDTA plasma(n=5) 85-103 97
heparin plasma(n=5) 90-105 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PECAM-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 90-96% 88-96% 92-100%
EDTA plasma(n=5) 89-98% 82-100% 82-97% 84-99%
heparin plasma(n=5) 88-97% 84-98% 80-99% 83-93%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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