Human OPN(Osteopontin) ELISA Kit

Manual
  • Specifications
Product name

Human OPN(Osteopontin) ELISA Kit

Catalogue No.

EH0248

Alias

OPN(Osteopontin) ELISA Kit, Eta-1 ELISA Kit, Spp1 ELISA Kit, BNSP ELISA Kit, Bone sialoprotein 1 ELISA Kit, MGC110940 ELISA Kit, Nephropontin ELISA Kit, secreted phosphoprotein-1(osteopontin, bone sialoprotein) ELISA Kit, SPP-1 ELISA Kit, SPP1 ELISA Kit, CALPHA1 fusion ELISA Kit, Urinary stone protein ELISA Kit, uropontin ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

OPN ELISA Kit allows for the in vitro quantitative determination of OPN concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

156-10000pg/ml

Sensitivity

< 93.75pg/ml

Standard curve

Human OPN(Osteopontin) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of OPN and the recovery rates were calculated by comparing the measured value to the expected amount of OPN in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-103 95
EDTA plasma(n=5) 91-101 97
heparin plasma(n=5) 87-104 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OPN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-101% 87-104% 85-98% 88-102%
EDTA plasma(n=5) 82-96% 86-101% 88-101% 87-101%
heparin plasma(n=5) 86-99% 82-98% 81-96% 82-86%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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Hotline

027-87384275
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