Human MITF(Microphthalmia-associated transcription factor) ELISA Kit

Manual
  • Specifications
Product name

Human MITF(Microphthalmia-associated transcription factor) ELISA Kit

Catalogue No.

EH0947

Alias

MITF ELISA Kit, BHLHE32(Microphthalmia-associated transcription factor) ELISA Kit, MI ELISA Kit, WS2A ELISA Kit, Waardenburg syndrome, type 2A ELISA Kit, Class E basic helix-loop-helix protein 32 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

MITF ELISA Kit allows for the in vitro quantitative determination of MITF concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.625-40ng/ml

Sensitivity

< 0.375ng/ml

Standard curve

Human MITF(Microphthalmia-associated transcription factor) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MITF and the recovery rates were calculated by comparing the measured value to the expected amount of MITF in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-103 96
EDTA plasma(n=5) 86-101 95
heparin plasma(n=5) 85-102 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MITF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-102% 92-100% 87-105% 92-104%
EDTA plasma(n=5) 83-99% 82-101% 84-99% 87-100%
heparin plasma(n=5) 87-96% 81-100% 83-91% 80-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Service

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Hotline

027-87384275
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