Human IL1R1(Interleukin 1 Receptor Type I) ELISA Kit

Manual
  • Specifications
Product name

Human IL1R1(Interleukin 1 Receptor Type I) ELISA Kit

Catalogue No.

EH0186

Alias

IL1R1(Interleukin 1 Receptor Type I) ELISA Kit, IL-1R1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IL1R1 ELISA Kit allows for the in vitro quantitative determination of IL1R1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human IL1R1(Interleukin 1 Receptor Type I) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL1R1 and the recovery rates were calculated by comparing the measured value to the expected amount of IL1R1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-95 91
EDTA plasma(n=5) 89-103 97
heparin plasma(n=5) 87-102 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1R1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-100% 91-104% 88-99% 88-104%
EDTA plasma(n=5) 82-99% 87-95% 82-100% 83-98%
heparin plasma(n=5) 80-100% 80-91% 80-99% 84-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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