Human IL-1beta(Interleukin 1 Beta) ELISA Kit

Manual
  • Specifications
Product name

Human IL-1beta(Interleukin 1 Beta) ELISA Kit

Catalogue No.

EH0185

Alias

IL-1β ELISA Kit, IL-1beta ELISA Kit, IL1B ELISA Kit, IL1-BETA ELISA Kit, IL1F2 ELISA Kit, catabolin ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IL-1beta ELISA Kit allows for the in vitro quantitative determination of IL-1beta concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

3.9-250pg/ml

Sensitivity

< 2.344pg/ml

Standard curve

Human IL-1beta(Interleukin 1 Beta) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-1beta and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1beta in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-101 94
EDTA plasma(n=5) 85-102 94
heparin plasma(n=5) 92-96 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1beta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-104% 90-101% 92-103% 87-100%
EDTA plasma(n=5) 82-99% 88-98% 87-99% 84-101%
heparin plasma(n=5) 80-98% 80-97% 80-100% 80-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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Hotline

027-87384275
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