Human IL-12(p40)(Interleukin 12 p40) ELISA Kit

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Product name

Human IL-12(p40)(Interleukin 12 p40) ELISA Kit

Catalogue No.

EH0175

Alias

IL-12 (p40) ELISA Kit, IL12B ELISA Kit, IL-12B ELISA Kit, CLMF2 ELISA Kit, NKSF2 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-12 (p40) and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12 (p40) in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10493
EDTA plasma(n=5)85-10599
heparin plasma(n=5)86-10395
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12 (p40) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)88-103%85-103%90-95%85-105%
EDTA plasma(n=5)84-100%84-90%87-99%83-101%
heparin plasma(n=5)80-98%83-95%87-99%80-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Current product's manual: Manual

Protocol may be updated. Please feel free to contact Finetest Team to acquire the latest version.

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