Human IL-12(p40)(Interleukin 12 p40) ELISA Kit

Manual
  • Specifications
Product name

Human IL-12(p40)(Interleukin 12 p40) ELISA Kit

Catalogue No.

EH0175

Alias

IL-12(p40) ELISA Kit, IL12B ELISA Kit, IL-12B ELISA Kit, CLMF2 ELISA Kit, NKSF2 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IL-12 ELISA Kit allows for the in vitro quantitative determination of IL-12(p40) concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human IL-12(p40)(Interleukin 12 p40) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-12(p40) and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12(p40) in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-104 93
EDTA plasma(n=5) 85-105 99
heparin plasma(n=5) 86-103 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12(p40) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-103% 85-103% 90-95% 85-105%
EDTA plasma(n=5) 84-100% 84-90% 87-99% 83-101%
heparin plasma(n=5) 80-98% 83-95% 87-99% 80-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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