Human IL-12 (Interleukin 12) ELISA Kit

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Product name

Human IL-12 (Interleukin 12) ELISA Kit

Catalogue No.

EH0176

Alias

Interleukin 12 p70 ELISA Kit, CLMF p35 ELISA Kit, Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit, IL-12 subunit p35 ELISA Kit, IL-12, subunit p35 ELISA Kit, interleukin 12, p35 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Standard curve
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-12 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)89-10597
EDTA plasma(n=5)86-10394
heparin plasma(n=5)85-9590
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)86-98%85-103%87-101%93-105%
EDTA plasma(n=5)83-92%82-96%91-97%83-101%
heparin plasma(n=5)83-97%80-100%83-95%81-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Current product's manual: Manual

Protocol may be updated. Please feel free to contact Finetest Team to acquire the latest version.

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Service

Hotline

027-87384275
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