Human IFNγ(Interferon gamma) ELISA Kit

Manual
  • Specifications
Product name

Human IFNγ(Interferon gamma) ELISA Kit

Catalogue No.

EH0164

Alias

IFN-γ ELISA Kit, IFNG ELISA Kit, IFG ELISA Kit, IFI ELISA Kit, Type II Interferon ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IFNγ ELISA Kit allows for the in vitro quantitative determination of IFNγ concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Standard curve

Human IFNγ(Interferon gamma) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IFNγ and the recovery rates were calculated by comparing the measured value to the expected amount of IFNγ in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-101 93
EDTA plasma(n=5) 87-101 96
heparin plasma(n=5) 87-102 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFNγ and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-101% 86-101% 87-102% 85-100%
EDTA plasma(n=5) 89-101% 83-94% 86-92% 88-101%
heparin plasma(n=5) 95-99% 81-86% 80-97% 80-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Service

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Hotline

027-87384275
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