Human HTT(Huntingtin) ELISA Kit

Manual
  • Specifications
Product name

Human HTT(Huntingtin) ELISA Kit

Catalogue No.

EH1336

Alias

HTT ELISA Kit, Huntingtin ELISA Kit, Huntington disease protein ELISA Kit, HD protein ELISA Kit, HD ELISA Kit, IT15 ELISA Kit

Detection method

Competitive ELISA, Coated with Antibody

Application

HTT ELISA Kit allows for the in vitro quantitative determination of HTT concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.6-1000pg/ml

Sensitivity

< 9.375pg/ml

Standard curve

Human HTT(Huntingtin) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of HTT and the recovery rates were calculated by comparing the measured value to the expected amount of HTT in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-102 95
EDTA plasma(n=5) 86-105 95
heparin plasma(n=5) 85-104 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HTT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-99% 85-105% 85-101% 87-97%
EDTA plasma(n=5) 82-101% 82-101% 88-100% 87-98%
heparin plasma(n=5) 84-100% 80-94% 80-99% 80-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Service

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Hotline

027-87384275
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