Human HTR1B(5-hydroxytryptamine receptor 1B) ELISA Kit

Manual
  • Specifications
Product name

Human HTR1B(5-hydroxytryptamine receptor 1B) ELISA Kit

Catalogue No.

EH1152

Alias

HTR1B ELISA Kit, 5-HT1B(5-hydroxytryptamine receptor 1B) ELISA Kit, 5HT1DB ELISA Kit, HTR1D2 ELISA Kit, HTR1DB ELISA Kit, S12 ELISA Kit, Serotonin 1D beta receptor ELISA Kit, Serotonin receptor 1B ELISA Kit, 5-HT-1B ELISA Kit, 5-HT1DB ELISA Kit, 5-HT-1D-beta ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

HTR1B ELISA Kit allows for the in vitro quantitative determination of HTR1B concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78-5000pg/ml

Sensitivity

< 46.9pg/ml

Standard curve

Human HTR1B(5-hydroxytryptamine receptor 1B) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of HTR1B and the recovery rates were calculated by comparing the measured value to the expected amount of HTR1B in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-100 96
EDTA plasma(n=5) 91-98 94
heparin plasma(n=5) 89-104 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HTR1B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-98% 85-105% 85-93% 95-105%
EDTA plasma(n=5) 88-101% 82-101% 83-99% 90-101%
heparin plasma(n=5) 84-100% 83-99% 80-97% 85-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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