Human HSP-70(Heat Shock Protein 70) ELISA Kit

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  • Specifications
Product name

Human HSP-70(Heat Shock Protein 70) ELISA Kit

Catalogue No.

EH3242

Alias

HSP-70(Heat Shock Protein 70) ELISA Kit, HSPA1A ELISA Kit, HSP70 ELISA Kit, HSP70-1A ELISA Kit, HSP72 ELISA Kit, HSPA1A ELISA Kit, dnaK-type molecular chaperone HSP70-1 ELISA Kit, heat shock 70kD protein 1A ELISA Kit, heat shock-induced protein ELISA Kit, HSP70-1 ELISA Kit, HSP70-2 ELISA Kit, HSP70I ELISA Kit, HSPA1 ELISA Kit, HSPA1B ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

HSP-70 ELISA Kit allows for the in vitro quantitative determination of HSP-70 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of HSP-70 and the recovery rates were calculated by comparing the measured value to the expected amount of HSP-70 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-105 98
EDTA plasma(n=5) 90-102 94
heparin plasma(n=5) 89-100 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSP-70 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-102% 90-105% 89-105% 95-104%
EDTA plasma(n=5) 84-100% 87-101% 83-100% 84-96%
heparin plasma(n=5) 82-100% 84-100% 80-100% 84-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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