Human GPT(Alanine aminotransferase 1) ELISA Kit

Manual
  • Specifications
Product name

Human GPT(Alanine aminotransferase 1) ELISA Kit

Catalogue No.

EH0770

Alias

GPT ELISA Kit, ALT1(Alanine aminotransferase 1) ELISA Kit, GPT 1 ELISA Kit, AAT1 ELISA Kit, Glutamic–pyruvic transaminase 1 ELISA Kit, Glutamate pyruvate transaminase 1 ELISA Kit, Glutamic–alanine transaminase 1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

GPT ELISA Kit allows for the in vitro quantitative determination of GPT concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

3.12-200mIU/ml

Sensitivity

< 1.875mIU/ml

Standard curve

Human GPT(Alanine aminotransferase 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of GPT and the recovery rates were calculated by comparing the measured value to the expected amount of GPT in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-105 95
EDTA plasma(n=5) 87-99 96
heparin plasma(n=5) 87-100 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-103% 88-97% 89-103% 86-104%
EDTA plasma(n=5) 92-99% 83-97% 83-98% 92-101%
heparin plasma(n=5) 81-98% 81-98% 80-97% 80-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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Hotline

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